Capsaicin Inhibits Laryngeal Cancer Cells Growth in vivo and in vitro through Downregulating the PI3K/Akt Pathway
1 Department of Otolaryngology-Head and Neck Surgery, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, 434020, China
2 Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China
* These authors contributed equally to this work
Cancer Genetics and Epigenetics, 2019, Vol. 7, No. 2
Received: 15 Feb., 2019 Accepted: 12 Apr., 2019 Published: 11 Jun., 2019
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Capsaicin obviously restrains the progress of a lot of cancers. But, its role is not clear in laryngeal cancer. In vitro and in vivo the task of this study was to survey the inhibition of Hep-2 laryngeal cancer cell because of capsaicin. The Hep-2 laryngeal cancer cell line was treated in 0 μM, 100 μM, 200 μM and 300 μM different concentrations of capsaicin. Then we detected 0, 24, 48 and 72 h cell apoptosis, and the study probed in vitro the grade in proteins of PI3K/Akt pathway-related. The study divided the BALB/c nude mice to three groups: they are prevention group, treatment group and control group. In vivo, in every group we inoculated the nude mice with Hep-2 cells, and we feed mice in prevention and treatment groups in 0.5% capsaicin feed. We investigated the tumoricidal effect of capsaicin in nude mice, and we detected each group nude mice the expressions of PI3K/Akt pathway-related proteins in tumors after eight weeks. When the concentration of the capsaicin is increased, we found that the cell proliferation was decreased and the apoptosis was increased obviously. We found the grades of pathway-related proteins PI3K/Akt were reduced. The study showed in mice experiments the tumors were smaller in the prevention group and treatment group, and we can find when compared to control group, the prevention and treatment groups were reduced in PI3K/Akt pathway-related proteins. At the same time, we found the heart, liver and kidney tissues in all rats were no changes in HE staining. In conclusion, the study showed in vivo and in vitro the growth of Hep-2 cells were inhibited by capsaicin and the apoptosis was induced by capsaicin effectively. The result probably be the PI3K/Akt pathway was suppressed by the capsaicin.
Laryngeal cancer; Capsaicin; Apoptosis; PI3K/Akt pathway
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Cancer Genetics and Epigenetics
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